The increasing worldwide incidence of colon cancer has been linked to obesity and consumption of a high-fat Western diet. To test the hypothesis that a high-fat diet (HFD) promotes colonic aberrant crypt (AC) formation in a manner associated with gut bacterial dysbiosis， we examined the susceptibility to azoxymethane (AOM)-induced colonic AC and microbiome composition in C57/BL6 mice fed a modified AIN93G diet (AIN， 16% fat， energy) or an HFD (45% fat， energy) for 14 weeks. Mice receiving the HFD exhibited increased plasma leptin， body weight， body fat composition and inflammatory cell infiltration in the ileum compared with those in the AIN group. Consistent with the gut inflammatory phenotype， w... More
The increasing worldwide incidence of colon cancer has been linked to obesity and consumption of a high-fat Western diet. To test the hypothesis that a high-fat diet (HFD) promotes colonic aberrant crypt (AC) formation in a manner associated with gut bacterial dysbiosis， we examined the susceptibility to azoxymethane (AOM)-induced colonic AC and microbiome composition in C57/BL6 mice fed a modified AIN93G diet (AIN， 16% fat， energy) or an HFD (45% fat， energy) for 14 weeks. Mice receiving the HFD exhibited increased plasma leptin， body weight， body fat composition and inflammatory cell infiltration in the ileum compared with those in the AIN group. Consistent with the gut inflammatory phenotype， we observed an increase in colonic AC， plasma interleukin-6， tumor necrosis factor-α， monocyte chemoattractant protein-1 and inducible nitric oxide synthase in the ileum of the HFD-AOM group compared with the AIN-AOM group. Although the HFD and AIN groups did not differ in bacterial species number， the HFD and AIN diets resulted in different bacterial community structures in the colon. The abundance of certain short-chain fatty acid (SCFA) producing bacteria (e.g.， Barnesiella) and fecal SCFA (e.g.， acetic acid) content were lower in the HFD-AOM group compared with the AIN and AIN-AOM groups. Furthermore， we identified a high abundance of Anaeroplasma bacteria， an opportunistic pathogen in the HFD-AOM group. Collectively， we demonstrate that an HFD promotes AC formation concurrent with an increase of opportunistic pathogenic bacteria in the colon of C57BL/6 mice.
