| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The synthetic genes were custom synthesized by Genscript (Piscataway, NJ, USA) and cloned into the pCrEX1 nuclear expression vector (based on pSR108 [19]) using the restriction sites SapI (LguI) and BglII. Figure 1 gives an overview of the expression cassettes … | Get A Quote |
Dietary supplements and functional foods are becoming increasingly popular complements to regular diets. A recurring ingredient is the essential cofactor vitamin B (B). Microalgae are making their way into the dietary supplement and functional food market but do not produce B， and their B content is very variable. In this study， the suitability of using the human B-binding protein intrinsic factor (IF) to enrich bioavailable B using microalgae was tested. The IF protein was successfully expressed from the nuclear genome of the model microalga and the addition of an N-terminal ARS2 signal peptide resulted in efficient IF secretion to the medium. Co-abundance of B and the secreted IF suggests the algal produ... More
