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Computational design of generalist cyclopropanases with stereodivergent selectivity

Authors: Zhuofan Shen; Mary G. Siriboe; Xinkun Ren; Thakshila Dayananda; Jermaine L. Jenkins; Sagar D. Khare; Rudi Fasan

Journal: Nature Communications

PubMed ID: 41587967

Doi: 10.1038/s41467-026-68327-1

Computational design of generalist cyclopropanases with stereodivergent selectivity

Authors: Zhuofan Shen; Mary G. Siriboe; Xinkun Ren; Thakshila Dayananda; Jermaine L. Jenkins; Sagar D. Khare; Rudi Fasan

Journal: Nature Communications

PubMed ID: 41587967

Doi: 10.1038/s41467-026-68327-1

Catalytic Mechanism of SARS-CoV-2 3-Chymotrypsin-Like Protease as Determined by Steady-State and Pre-Steady-State Kinetics

Authors: Jiyun Zhu; Alexandria M. Kemp; Bala C. Chenna; Vivek Kumar; Andrew Rademacher; Sangho Yun; Arthur Laganowsky; Thomas D. Meek

Journal: ACS catalysis

PubMed ID: 39722883

Doi: 10.1021/acscatal.4c04695

Bioinformatic prediction and experimental validation of RiPP recognition elements

Authors: Kyle E. Shelton; Douglas A. Mitchell

Journal: Methods in enzymology

PubMed ID: 36682862

Doi: 10.1016/bs.mie.2022.08.050

Bioinformatic prediction and experimental validation of RiPP recognition elements

Authors: Kyle E. Shelton; Douglas A. Mitchell

Journal: Methods in enzymology

PubMed ID: 36682862

Doi: 10.1016/bs.mie.2022.08.050

Bioinformatic prediction and experimental validation of RiPP recognition elements

Authors: Kyle E. Shelton; Douglas A. Mitchell

Journal: Methods in enzymology

PubMed ID: 36682862

Doi: 10.1016/bs.mie.2022.08.050

Overexpression of PEX14 results in mistargeting to mitochondria, accompanied by organelle fragmentation and clustering in human embryonic kidney cells

Authors: Renate L M Jansen, Rinse de Boer, Eline M F de Lange, Janet Koster, Rifka Vlijm, Hans R Waterham, Ida J van der Klei

Journal: Biochim Biophys Acta Mol Cell Res

PubMed ID: 38762172

Doi: 10.1016/j.bbamcr.2024.119754

Products & Services: Molecular Biology Reagents

Deep mutational scanning reveals a correlation between degradation and toxicity of thousands of aspartoacylase variants

Authors: Martin Grønbæk-Thygesen, Vasileios Voutsinos, Kristoffer E. Johansson, Thea K. Schulze, Matteo Cagiada, Line Pedersen, Lene Clausen, Snehal Nariya, Rachel L. Powell, Amelie Stein, Douglas M. Fowler, Kresten Lindorff-Larsen & Rasmus Hartmann-Petersen

Journal: Nat Commun

PubMed ID: 38740822

Doi: 10.1038/s41467-024-48481-0

Products & Services: Mutagenesis Services

Biosynthetic production of anticoagulant heparin polysaccharides through metabolic and sulfotransferases engineering strategies

Authors: Jian-Qun Deng, Yi Li, Yu-Jia Wang, Ya-Lin Cao, Si-Yu Xin, Xin-Yu Li, Rui-Min Xi, Feng-Shan Wang & Ju-Zheng Sheng

Journal: Nat Commun

PubMed ID: 38704385

Doi: 10.1038/s41467-024-48193-5

Products & Services: Gene Synthesis

Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a

Authors: Kaizhao Chen, Wenjun Sun, Mingtian Zhong, Jiaqi Xie, Yongkun Huo, Xiuqi Lu, Ziting Chen, Bo Sun, Xingxu Huang, Xinjie Wang， Ming Liu , Xiaodong Ma, Peixiang Ma

Journal: Sensors and Actuators B: Chemical

Doi: 10.1016/j.snb.2024.135389

Products & Services: Protein Electrophoresis and Western