eSpCas9 mRNA(Cap1, m1Ψ)

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al Science. 2015 Dec 1. eSpCas9 can reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.

价格	￥1000
产品编号	RP-A00018-0.1
规格	0.1 mg 0.2 mg 1 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al., Science (2015 Dec 1). eSpCas9 significantly reduces off-target effects (by more than 10-fold), while maintaining high on-target genome editing efficiency.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

This construct offers high-precision genome editing with minimized off-target activity, making it ideal for CRISPR validation, therapeutic research, and functional genomics studies.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	4479nt
Full mRNA Molecular Weight	1457890
Storage buffer	1mM Sodium citrate, pH6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect a total of 0.5-1.5 µg of eSpCas9 mRNA and sgRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10⁵ cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.

图例

eSpCas9 mRNA and TRAC sgRNA (molar ratio 1:10) were transfected into 2×105 HEK293T cells using LNP. 48h after transfection, cells were collected to extract gDNA. Genome editing efficiency were verified by PCR, Sanger sequencing and ICE analysis. »

Expression of eSpCas9 mRNA in A549 cells. The eSpCas9 expression was measured 48 hours after transfection by ELISA. »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.