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eSpCas9 mRNA(Cap1, m1Ψ)

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al Science. 2015 Dec 1. eSpCas9 can reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
￥1000
RP-A00018

Description

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al., Science (2015 Dec 1). eSpCas9 significantly reduces off-target effects (by more than 10-fold), while maintaining high on-target genome editing efficiency.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA 
 
This construct offers high-precision genome editing with minimized off-target activity, making it ideal for CRISPR validation, therapeutic research, and functional genomics studies.
产品属性
Form Liquid
Concentration 1mg/mL
Full mRNA length 4479nt
Full mRNA Molecular Weight 1457890
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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质控参数
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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应用指导
Transfect a total of 0.5-1.5 µg of eSpCas9 mRNA and sgRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10⁵ cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.
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图例
ESpCas9 MRNA(Cap1, M1Ψ)

eSpCas9 mRNA and TRAC sgRNA (molar ratio 1:10) were transfected into 2×105 HEK293T cells using LNP. 48h after transfection, cells were collected to extract gDNA. Genome editing efficiency were verified by PCR, Sanger sequencing and ICE analysis. »

ESpCas9 MRNA(Cap1, M1Ψ)

Expression of eSpCas9 mRNA in A549 cells. The eSpCas9 expression was measured 48 hours after transfection by ELISA. »

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.