|Description||Green Taq DNA Polymerase is designed to increase the stability of the Taq enzyme for more convenient transport and applications. The polymerase (1000 U) is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction. It can be stable for six months if stored at 4°C or for up to one month without significant loss of activity at ambient temperature. Our technology can increase the stability of Green Taq DNA at higher temperature (72°C). The higher yield PCR product can be harvested in long PCR amplification.
PCR Reaction Buffer (with Mg 2+ ): 100 mM Tris-HCl, 15 mM MgCl 2 , 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C).
Enzyme Storage Buffer: 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C)
The applications of Green Taq DNA Polymerase include the following:
|Storage||This product is shipped at ambient temperatures for several weeks. Green Taq DNA Polymerase will remain stable for at least six months if stored at 4°C and for at least two years if stored at -20°C. Please refer to the product label for the specific shelf life of each batch.|
|Note||One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.|