内毒素检测&去除试剂盒
内毒素是革兰氏阴性菌细胞壁的一部分,是生物制品一个重要的污染物,可在宿主体内导致气相反应,如内毒素休克、组织损伤甚至死亡。FDA要求所有药物的最终配方必须进行内毒素检测。因此,内毒素检测对于用于人体或动物的生物制品、药品以及医疗设备都是非常有必要的。
金斯瑞ToxinSensor 内毒素检测系统使用FDA批准的鲎试剂测试方法来实现快速和高度敏感的细菌内毒素检测。鲎试剂法内毒素检测试剂盒能定量检测范围广泛的内毒素(0.01-1EU/ml)。凝胶内毒素检测试剂盒是一种快速定性试剂盒,可以快速检测呈阳性或阴性的结果。ToxinEraser 内毒素去除试剂盒在内毒素的去除过程中具有较高的结合能力(超过2000000EU/ml)。
*声明:内毒素检测试剂盒包装瓶/包装盒变更,点击查看详情说明文件。显色法检测试剂盒终止液组分停售,点击查看情况说明。
ToxinSensor 内毒素检测试剂盒
- 包含实验所需全部试剂
- 高度线性化和可重复的结果
- 高灵敏度:0.01EU/ml
ToxinSensor 内毒素检测试剂盒适用于内毒素体外试验,包括用于人体或动物的生物制品、药品以及医疗设备。
产品订购信息
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ToxinEraser 内毒素去除系统
—高效去除样品中的内毒素!- 高结合能力,至少2, 000, 000EU/ml (柱体积)
- 高再生能力(>90%)
- 高稳定性和高去除力:可将样品中内毒素含量降至0.1EU/ml*
*重复使用ToxinEraser 内毒素去除树脂,最终去除效率根据样品不同会有所区别。
金斯瑞ToxinEraser 是一种有效的内毒素去除工具,在重复使用之后可以将样品中内毒素水平降低至小于1EU/ mg。该试剂盒可以用于去除蛋白质、多肽、抗体或DNA样品中的内毒素。
产品订购信息
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选择指导
ToxinSensor 鲎试剂检测法试剂盒 |
ToxinSensor 凝胶检测法试剂盒 |
ToxinSensor 一次性检测试剂盒 |
|
---|---|---|---|
基本介绍 | 该试剂盒利用显色鲎试验定量检测范围广泛的内毒素 | 该试剂盒基于胶凝作用原理,是一个方便的内毒素定性检测试剂盒 | 该试剂盒用于一次性检测内毒素含量,有不同的灵敏度可供选择 |
应用范围 | 准确的检测内毒素含量 | 定性检测内毒素 | 一次性定性检测内毒素 |
灵敏度 | 0.01-1EU/ml | 0.25EU/ml | 0.03EU/ml-0.25EU/ml |
鲎试剂水 | 包含 | 包含 | 不包含 |
分光光度计 | 需要 | 不需要 | 不需要 |
试剂盒组分 |
即用试剂 以及无热源的移液枪头和试管 |
即用试剂 以及无热源的移液枪头和试管 |
即用试剂 不含无热源的耗材 |
包装 | L00350C 16次 L00350 32次 |
L00351 40次 | L00856-L00859 |
怎样为医疗器械检测内毒素?
为医疗器械管腔注入不含内毒素的水15毫升(如输液装置、透析管等),密封两端后在37°C水浴孵育2小时,然后将水转移至无内毒素的小瓶。使用ToxinSensor 鲎试剂检测法试剂盒(目录号L00350)检测水体中的内毒素浓度,在水中的总内毒素值便可以确定。
制备标准品溶液时能否使用塑料容器?
一般而言,内毒素在塑料表面的附着比玻璃表面更强,标准的实验室压力蒸汽灭菌对内毒素几乎不会有任何影响。如要使用玻璃器皿,建议在180°C过夜灭菌以破坏任何附加的内毒素分子。我们建议您仅使用新的塑料制品,并在准备标准品溶液时确认无内毒素。金斯瑞提供Toxinsensor无内毒素离心管(目录号M01072)用于样品处理。
标准品溶液能否重复使用?
标准品的母液是可以保存后使用的,但是不可以反复冻融。母液的保存期限请参考说明书。而不同浓度标准品的梯度稀释液是不可以保存后使用的,因为浓度太低,效价会降低,会影响线性。所以,标准品的稀释液建议每次实验时新鲜制备。
如何确定最终的数据的可靠性?
首先,建议制备所需实验试剂时在通风橱内进行以避免污染;
对于鲎试剂检测法试剂盒(目录号L00350),如果样品的OD值在标准范围内,最终的数据可以获得良好的线性(R≥0.98);
对于凝胶检测法试剂盒(目录号L00351),可以通过阴性和阳性对照,获得可靠的结果。如果在阴性对照下得到一个阳性的结果,这表明,鲎试剂的水可能已经被污染;如果阳性对照不凝胶,需要考虑标准品的涡旋和稀释操作以及孵育温度是否符合要求,也可能是鲎试剂保存不当,已经失去活性。是否可以使用96孔板读取545nm的吸光值?
鲎试剂检测法试剂盒(L00350)为高灵敏度定量设计,不适用于96孔板;但是可以在离心管中进行实验,在显色后将溶液转移至96孔板中进行读取。
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- Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
- Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
- Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
- Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
- A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
- Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
- Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
- K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
- Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
- Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
- Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
- Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
- Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
- Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
- Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
- Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
- Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.
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