内毒素检测&去除试剂盒

常见问题解答

• 检测过程需要用到哪些设备？

  ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350 & L00350C) 需要使用可设置 37 ℃ ± 1 ℃ 的水浴锅进行孵育，使用带有 545 nm 滤光片的分光光度计或酶标仪进行结果的读取；ToxinSensor™ Gel clot Endotoxin Assay Kit (Cat. No. L00351) 和 ToxinSensor™ Single Test Kits (Cat. No.L00856-L00859) 仅需要使用水浴锅或凝胶测定仪进行孵育即可。

• 能否用烘箱进行孵育？

  建议使用水浴或凝胶测定仪进行孵育，烘箱/摇床可能由于温度分布不均一导致孵育不充分。

• 哪些类型的样品与 ToxinSensor™ 内毒素检测系统不兼容？

  金斯瑞内毒素检测试剂盒不适用于人类疾病诊断、临床样本（例如抗生素样本）、患者管理和细胞/细菌培养基中的内毒素检测。此外，由于 β-葡聚糖会非特异性干扰鲎试剂并导致假阳性结果，我们不建议使用我们的内毒素检测试剂盒检测 β-葡聚糖样品或受 β-葡聚糖污染的样品。一般来说，在检测样品的内毒素水平之前，建议按照《中国药典》进行干扰测试。确保消除干扰因素。
  对于血液血清样品，可以用检查用水或样品处理液稀释10倍，再加热处理后进行检测。加热温度可以70℃，10min,也可根据样品进行调整。

• 样本怎么处理才能进行检测？

  根据药典要求，当供试品新建内毒素检测时须先进行内毒素干扰实验，或当供试品或鲎试剂的生产工艺或环境发生了可能影响实验结果的变化时需要进行内毒素干扰试验后再执行正式的检测。另外样本本身的pH对于内毒素检测准确性十分重要, 因此，如有必要，我们建议使用氢氧化钠 （0.1 M，溶解在 LAL 试剂水中） 或盐酸 （0.1 M，溶解在 LAL 试剂水中）调节 pH 值至6-8。

• 配制内毒素标准溶液时可以使用塑料器皿吗？

  相较于玻璃表面，内毒素更容易黏附在塑料制品表面，金斯瑞提供 ToxinSensor™ 无内毒素试管（Cat.No.: M01072）供您稀释或分装样品。

客户引文

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7. Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
8. Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
9. Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
10. Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
11. A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
12. Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
13. Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
14. K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
15. Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
16. Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
17. Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
18. Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
19. Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
20. Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
21. Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
22. Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
23. Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.
24. Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
25. Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
26. Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
27. Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
28. A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
29. Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
30. Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
31. K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
32. Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
33. Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
34. Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
35. Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
36. Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
37. Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
38. Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
39. Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
40. Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.