内毒素检测&去除试剂盒

ToxinEraser 内毒素去除系统

• 高结合能力，至少2, 000, 000EU/ml （柱体积）
• 高再生能力（>90%）
• 高稳定性和高去除力：可将样品中内毒素含量降至0.1EU/ml^*

^*重复使用ToxinEraser 内毒素去除树脂，最终去除效率根据样品不同会有所区别。

金斯瑞ToxinEraser 是一种有效的内毒素去除工具，在重复使用之后可以将样品中内毒素水平降低至小于1EU/ mg。该试剂盒可以用于去除蛋白质、多肽、抗体或DNA样品中的内毒素。

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选择指导

1. 怎样为医疗器械检测内毒素？

   为医疗器械管腔注入不含内毒素的水15毫升（如输液装置、透析管等），密封两端后在37°C水浴孵育2小时，然后将水转移至无内毒素的小瓶。使用ToxinSensor 鲎试剂检测法试剂盒（目录号L00350）检测水体中的内毒素浓度，在水中的总内毒素值便可以确定。

2. 制备标准品溶液时能否使用塑料容器？

   一般而言，内毒素在塑料表面的附着比玻璃表面更强，标准的实验室压力蒸汽灭菌对内毒素几乎不会有任何影响。如要使用玻璃器皿，建议在180°C过夜灭菌以破坏任何附加的内毒素分子。我们建议您仅使用新的塑料制品，并在准备标准品溶液时确认无内毒素。金斯瑞提供Toxinsensor无内毒素离心管（目录号M01072）用于样品处理。

3. 标准品溶液能否重复使用？

   标准品的母液是可以保存后使用的，但是不可以反复冻融。母液的保存期限请参考说明书。而不同浓度标准品的梯度稀释液是不可以保存后使用的，因为浓度太低，效价会降低，会影响线性。所以，标准品的稀释液建议每次实验时新鲜制备。

4. 如何确定最终的数据的可靠性？

   首先，建议制备所需实验试剂时在通风橱内进行以避免污染；
   对于鲎试剂检测法试剂盒（目录号L00350），如果样品的OD值在标准范围内，最终的数据可以获得良好的线性（R≥0.98）；
   对于凝胶检测法试剂盒（目录号L00351），可以通过阴性和阳性对照，获得可靠的结果。如果在阴性对照下得到一个阳性的结果，这表明，鲎试剂的水可能已经被污染；如果阳性对照不凝胶，需要考虑标准品的涡旋和稀释操作以及孵育温度是否符合要求，也可能是鲎试剂保存不当，已经失去活性。

5. 是否可以使用96孔板读取545nm的吸光值？

   鲎试剂检测法试剂盒（L00350）为高灵敏度定量设计，不适用于96孔板；但是可以在离心管中进行实验，在显色后将溶液转移至96孔板中进行读取。

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• Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
• Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
• Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
• Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
• A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
• Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
• Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
• K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
• Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
• Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
• Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
• Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
• Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
• Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
• Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
• Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
• Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.