| ￥2980 在线订购包邮(港澳台除外) | |
| L01109 | |
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| Product Description | Recombinant factor C endotoxin detection kit can replace the traditional limulus amebocyte lysate (LAL) for human and animal injection drugs (such as chemicals, radiopharmaceuticals, antibiotics, biological products, etc) and medical devices (such as dialysate, implantable devices, etc) raw and auxiliary materials, intermediate products, released products endotoxin detection. This kit is a highly sensitive, highly specific, stable and sustainably available alternative to limulus amebocyte lysate that does not depend on animal-derived components. It can measure endotoxin concentrations in the range of 0.005-5 EU/mL. | |||||||||||||||||||||||||||
| Detection Range | 0.005-5 EU/mL | |||||||||||||||||||||||||||
| Storage | The unopened kit is stable for 6 months at 2°C to 8°C.The control standard endotoxin is stored at 2°C to 8°C after dissolution, and the validity period is stable for 8 weeks. | |||||||||||||||||||||||||||
| Kit Contents |
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| Assay Principle | The assay was performed in a 96-well plate, and the fluorescence values were measured at an excitation/emission wavelength of 380/440nm at zero hour after sample addition and one hour after incubation at 37°C±1°C.The net fluorescence difference is obtained by calibrating the fluorescence reading difference (ΔRFU) between the control standard endotoxin and the sample using the fluorescence difference (ΔRFU) of the negative control. The logarithm of the net fluorescence difference is proportional to the logarithm of the endotoxin concentration and is linear over the range 0.005 to 5.0 EU/mL. The endotoxin concentration in the sample can be calculated according to the standard curve. |
Recombinant Factor C Endotoxin Detection Kit standard curve, and the correlation coefficient R2=0.999. »
The recovery rates of endotoxin spiked into 5 different biological preparations were evaluated, and the results indicated that the GenScript kit provided the most accurate recovery. »
The well-to-well precision across three batches of kits shows a CV<10%. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
