蛋白质研究和抗体纯化用磁珠
为了能够快速有效地纯化抗体和标签蛋白，金斯瑞（GenScript）提供了超顺磁性的MagBeads系列磁珠。GenScript MagBeads偶联有抗标签抗体、重组蛋白、多肽和小分子金属螯合试剂配基，可与抗体和带标签的蛋白以及Biotin标签分子特异性地结合。
磁株分离纯化方法可直接从细胞裂解物或细胞上清中纯化目的抗体和蛋白，减少了离心和过滤步骤，提高了纯化效率。高结合能力，高结合特异性和超快的磁响应特性，使得金斯瑞磁珠纯化产品非常适合快速纯化和高效浓缩目标产品，得到高得率和高纯度的纯化样品。
| 点击免费申请试用 |
|---|
|
请编写"产品编号+单位+姓名+手机号" 或直接拨打客服热线400-025-8686转5810 |
应用领域
- 抗体纯化
- 标签蛋白纯化，如：His，GST，Flag和生物素等
- 高通量抗体和标签蛋白的纯化
Protein A、Protein G和Protein A/G 磁珠
标签蛋白纯化磁珠
抗体磁珠纯化相关溶液
| 产品名称 | 产品编号 | 规格 | 订购 |
|---|---|---|---|
| AmMag™ Wash buffer for antibody purification | B00045 | 210g/包*，干粉 | 询价 |
*每包干粉可以配置成2 L缓冲溶液。
| AmMag™ Protein A Magnetic beads 动态载量 (L00695) | ||||
| Sample concentration (hIgG) | RT / 2 H | 37°C / Overnight | 4°C / 2 H | 4°C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 5 | 2 | 2.5mg/ml |
| 0.1mg/ml | 20mg/ml | 30mg/ml | 15 | 20 |
| 0.5mg/ml | 35mg/ml | 60mg/ml | 30mg/ml | 50mg/ml |
| 1mg/ml | 45mg/ml | 70mg/ml | 40 | 60 |
| 3mg/ml | 70mg/ml | 100mg/ml | 60 | 85 |
| Ni charged Magbeads 动态载量 (L00295) | ||||
| Sample concentration (MBP) | RT / 2 H | 37C / Overnight | 4C / 2 H | 4C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 2.5mg/ml | ||
| 0.1mg/ml | 30mg/ml | 20mg/ml | 10mg/ml | 20mg/ml |
| 0.5mg/ml | 50mg/ml | 40mg/ml | 30mg/ml | 40mg/ml |
| 2mg/ml | 60mg/ml | 50mg/ml | 40mg/ml | 50mg/ml |
| AmMag™Ni Magnetic Beads动态载量 (L00776) | |
| Sample concentration (MBP) | RT / 1 H |
|---|---|
| 0.01mg/ml | 1-2mg/ml |
| 0.1mg/ml | 8mg/ml |
| 0.5mg/ml | 9mg/ml |
| 1mg/ml | 9mg/ml |
| 应用 | 产品名称 | 原理 | 结合能力 | 规格 | 产品编号 |
|---|---|---|---|---|---|
| 抗体纯化 | Protein A 磁珠 | 通过重组蛋白A或G，与不同物种IgG及其亚基的结合特性 | 10mg兔 IgG/ml磁珠 | 4ml | L00273 |
| Protein A MagBeads MX 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00672 | ||
| AmMag™ 耐碱Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00695 | ||
| Protein G 磁珠 | 10mg山羊 IgG/ml磁珠 | 2ml | L00274 | ||
| Protein G MagBeads MX 磁珠 | 25mg人 IgG/ml磁珠 | 4ml | L00673 | ||
| Protein A/G 磁珠 | 10mg山羊或兔 IgG/ml磁珠 | 2ml | L00277 | ||
| Protein A/G Magbeads MX 磁珠 | 30mg 人lgG/ml磁珠 | 2ml | L00894 | ||
| His标签蛋白纯化 | Ni-charged 磁珠 | 通过Ni2+捕获His标签蛋白 | 40mg 6×His标签蛋白 (27kD)/ml磁珠 | 8ml | L00295 |
| AmMag™ Ni Magnetic beads | 通过带镍电荷的TED配体捕获His标签蛋白，耐EDTA和DTT | 10mg 6×His标签蛋白 (27kD)/ml磁珠 | 4/40/400ml | L00776 | |
| Flag标签蛋白纯化 | MonoRab™ Anti-DYKDDDDK Magnetic Beads | 通过DYKDDDDK兔单抗捕获Flag标签蛋白 | 1.5mg DYKDDDDK标签蛋白（50KDa）/ml磁珠 | 1/5/25ml | L00835 |
| GST融合蛋白纯化 | GST磁珠 | 通过还原型谷胱甘肽捕获GST融合蛋白 | 20 - 30mg GST/ml磁珠 | 8ml | L00895 |
| 生物素化样品分离纯化 | Streptavidin 磁珠 | 通过Streptavidin捕获生物素化的样品 | > 60nmol游离生物素/ml磁珠 | 2ml | L00936 |
- Y Kuroda., et al. Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3. J Pharm Biomed Anal. 2013 Mar 25;76:81-6.
- Hsieh YH., et al. Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Carcinogenesis.. 2013 Feb;34(2):475-85.
- Ferreira E., et al. Protein Interaction Studies Point To New Functions For Escherichia Coli Glyceraldehyde-3-Phosphate Dehydrogenase.Res Microbiol. 2013 Feb-Mar;164(2):145-54.
- Tyler RE., et al. Unassembled CD147 is an endogenous endoplasmic reticulum-associated degradation substrate. Mol Biol Cell. 2012 Dec;23(24):4668-78.
- Wiltrout E., et al. Misacylation Of Trna With Methionine In Saccharomyces Cerevisiae. Nucleic Acids Res. 2012 Nov 1;40(20):10494-506.
- Priyanka Sivadas, et al. A Flagellar A-Kinase Anchoring Protein With Two Amphipathic Helices Forms A Structural Scaffold In The Radial Spoke Complex. J Cell Biol. 2012 Nov 12;199(4):639-51.
- Dai C., et al.Loss Of Tumor Suppressor Nf1 Activates Hsf1 To Promote Carcinogenesis. J Clin Invest. 2012Oct 1;122(10):3742-54.
- Leyme A., et al. Identification Of Ilk As A New Partner Of The Adam12 Disintegrin And Metalloprotease In Cell Adhesion And Survival. Mol Biol Cell. 2012 Sep;23(17):3461-72.
- Dai MS., et al. Physical and functional interaction between ribosomal protein L11 and
the tumor suppressor ARF.
J Biol Chem. 2012 May 18;287(21):17120-9. - Tang X., et al. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen. 2012 Mar;17(3):314-26.
- An J., et al. CHCM1/CHCHD6, Novel Mitochondrial Protein Linked to Regulation of Mitofilin and Mitochondrial Cristae Morphology. J Biol Chem. 2012 Mar 2;287(10):7411-26.
- Kazuhiro Ishiguro, et al. Cutting Edge: Tubulin α Functions as an Adaptor in NFAT-importin β Interaction. J Immunol. 2011Mar 1;186(5):2710-3.
- Luo MH., et al. Human Cytomegalovirus Infection Causes Premature and Abnormal Differentiation of Human Neural Progenitor Cells. J Virol. 2010 Apr;84(7):3528-41.
- Dongzhu Ma, et al.Rheb GTPase Controls Apoptosis by Regulating Interaction of FKBP38 with Bcl-2 and Bcl-XL. J Biol Chem. 2010 Mar 19;285(12):8621-7.
- Crystal Morales, et al. Drosophila Glycoprotein 93 Is an Ortholog of Mammalian Heat Shock Protein gp96 (grp94, HSP90b1, HSPC4) and Retains Disulfide Bond-Independent Chaperone Function for TLRs and Integrins. J Immunol. 2009 Oct 15;183(8):5121-8.
- Cross BC., et al. Dissecting the physiological role of selective
transmembrane-segment retention at the ER translocon.
J Cell Sci. 2009 Jun 1;122(Pt 11):1768-77.
