Genscript Advanced Primer设计工具

** This online tool designs PCR primers for you.

Primer Name

Number To Return

Sequence

Left primer

Hybridization  probe

Right primer

Left Primer Sequence Input

Right Primer Sequence Input

Targets

Included Regions

Excluded Regions

Start Codon Position

Sequence Quality

Min Sequence Quality

Min End Sequence Quality

Sequence Quality Range Min

Sequence Quality Range Max

Primer Condition Details

	Min	Opt	Max
Primer Size
Primer Tm
Primer GC%

Max Tm Difference		Max Self Comp
Max #N's		Max 3's Self Comp
Max Poly-X		GC Clamp
Inside Target Penalty		Outside Target Penalty
First Base Index		Salt Concentration
Annealing Oligo Concentration		Max 3'Stability
Max Mispriming		Pair Max Mispriming

Primer Product Details

	Min	Opt	Max
Product Size
Product Tm

Objective Function Penalty Weights for Primers

Tm	Lt		Gt
Size	Lt		Gt
GC%	Lt		Gt

Self Complementarity
3' Self Complementarity
#N's
Mispriming
Sequence Quality
End Sequence Quality
Position Penalty
End Stability

Objective Function Penalty Weights for Primer Pairs

Product Size	Lt		Gt
Product Tm	Lt		Gt

Tm Difference
Any Complementarity
3' Complementarity
Pair Mispriming
Primer Penalty Weight
Hyb Oligo Penalty Weight

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