• 应用:
  Dot; Western blot; ELISA; Other applications;
• 浓度
  1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
• 溶解方法
  Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
• 描述
  Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
• 图片:
  

  [2 Images]

• 应用:
  Dot; Western blot; ELISA; Other applications;
• 浓度
  0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
• 溶解方法
  Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
• 描述
  Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
• 图片:
  

  [2 Images]

• 抗原物种:
  Peptostreptococcus magnus
• 应用:
  Flow cytometry analysis;
• 表达系统:
  E. coli
• 偶物联:
  iFluor488
• 图片:
  

  [2 Images]

• 抗原物种:
  Peptostreptococcus magnus
• 应用:
  Flow cytometry analysis;
• 表达系统:
  E. coli
• 偶物联:
  FITC
• 图片:
  

  [2 Images]

• 抗原物种:
  Peptostreptococcus magnus
• 应用:
  Flow cytometry analysis;
• 表达系统:
  E. coli
• 偶物联:
  iFluor647
• 图片:
  

  [2 Images]

9 Citations

• 描述
  GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg Human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
• 图片:
  

  [1 Image]

• 应用:
  WB; IHC; ICC/IF; IHF; FCM;
• 亚型:
  Rabbit IgG
• 偶物联:
  Unconjugated
• 免疫原:
  A synthesized peptide derived from human UBA52