Human

HEK 293

OX40/TNFRSF4 (Leu29-Ala216) Accession # P43489	Poly-His
N-term	C-term

ACT-135; ACT35 antigen; ACT35; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35

GenCrispr Cas9-C-NLS nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein with a C-terminal nucleic localization signal (NLS) that can be used for genome editing by inducing site-specific DNA double stranded breaks. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS. Compared with the mRNA or plasmid systems, transcription and translation processes are not required. This DNA-free system avoids the risk of inserting foreign DNA into the genome, which can be quite useful for gene editing-based disease therapy. Our highly pure and active Cas9 nuclease meets all of the researcher’s requirements (e.g. in vitro cleavage assay, RNP complex transfection, micro injection). Product Source: GenCrispr Cas9-C-NLS is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a C terminal nuclear localization signal (NLS). Key features: DNA-free: no external DNA added to system. High cleavage efficiency: NLS ensures the entry of Cas9 protein into nuclei. Low off target: transient expression of Cas9 nuclease. Time-saving: no need for transcription and translation. With this Cas9 Nuclease, you can: Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage. In vivo gene editing combined with specific gRNA by electroporation or injection.

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6C focuses on enhancing the performance of prime editing in transgenic rice plants. This specific variant of the prime editing technology is tailored to address the challenges of genetic improvement in crops, contributing to enhanced editing precision and efficiency in agricultural applications. The effectiveness of PE6c stems from its design improvements that enable it to facilitate targeted insertions and modifications in plant genomes.

WB; ICC/IF;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human Focal adhesion kinase 1 around the phosphorylation site of Y397

WB; IP;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human c Jun around the phosphorylation site of T91

WB; ICC/IF;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human FOXA2

WB; ICC/IF; IP;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human IRAK1

WB; FCM;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human MSH2

WB; IHC;

Rabbit IgG

Unconjugated

A synthesized peptide derived from mouse ICAM 2

WB; ICC/IF; FCM;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human SART1