CHO-K1/Spike/LTR-Luc
CHO-K1 cells overexpressing Spike protein and luciferase driven by HIV-1 LTR for application of binding screening or cell fusion assay
| 询价 | |
| RD00902 | |
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| 询价 | |
| RD00902 | |
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| Product Description | CHO-K1 cells overexpressing Spike protein and luciferase driven by HIV-1 LTR for application of binding screening or cell fusion assay |
| Paired Cat. No | RD00885 |
| Culture Properties | Adherent |
| Product Type | Cell pool |
| Size | 2 vials of frozen cells (>1X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | F-12K, 10% FBS, 8 μg/mL Puromycin, 400 μg/mL Hygromycin B |
| Complete Growth Medium | F-12K, 20% FBS |
| Freeze Medium-DATA | 90% Complete Growth Medium, 10% DMSO |
Flow cytometric analysis of CHO-K1/Spike/LTR-Luc stained with ACE2 Protein (Sino Biological, Cat. No.: 10108-H05H) after cell digestion using Accutase Cell Dissociation Reagent. »
CHO-K1/Spike/LTR-Luc cell pool cells were plated and incubated at 37°C, 5% CO2 for 16-20 hours before the addition of increasing concentrations of anti-Spike neutralizing antibody, followed by 1-hour incubation. Subsequently, HEK293/ACE2/Tat cell pool cells were added. After a 4-hour incubation, luciferase substrate reagent was introduced, and luminescence was measured using the PHERAstar system. The data were analyzed using GraphPad Prism® software. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
