Human

CHO

CD19 (Pro20-Lys291) Accession # P15391	hFc
N-term	C-term

CD19; Leu-12; B4; B-lymphocyte antigen

Mouse

CHO

IL-7 (Glu26-Ile154) Accession # Q544C8	Poly-His
N-term	C-term

IL7; IL-7; interleukin 7; Lymphopoietin 1; LP-1; pre-B cell factor

Human

CHO

Acrp30 (Glu19-Asn244) Accession # Q15848

Acrp-30; GBP-28; Apm-1

WB; IHC; IP;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human p70 S6K1

WB; IHC; ICC/IF; IHF; FCM;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human alpha Smooth muscle actin

WB; IHC;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human COPA

WB; IP;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human MNX1

WB; IHC; ICC/IF; IHF;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human DNA PKcs

WB; IHC; ICC/IF; IHF; IP; FCM;

Rabbit IgG

Unconjugated

A synthesized peptide derived from human YBX1

GenCrispr NLS-Cas9-EGFP is a fusion protein developed by GenScript. It contains a nuclear localization sequence (NLS) on its N terminal and EGFP on the C terminal. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The Cas9 RNP complex can localize to the nucleus immediately upon entering the cell with the addition of a nuclear localization signal (NLS). There is no requirement for transcription and translation compared with mRNA or plasmid systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cells minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). The EGFP can be used as a reporter for tracking or sorting transfected cells, which enables the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (FACS). It significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications.