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| Protein Electrophoresis and Western | P0012). 5X SDS loading buffer (Beyotime, P0015L) was then added to the lysates. Proteins were separated by electrophoresis on 4% 20% SurePAGETM gels (GenScript) and transferred to polyvinylidene fluoride membranes (Millipore, IPVH00010) using an eBlot (GenScript) wet transfer system. The membranes were blocked by electrophoresis on 4% 20% SurePAGETM gels (GenScript) and transferred to polyvinylidene fluoride membranes (Millipore, IPVH00010) using an eBlot (GenScript) wet transfer system. The membranes were blocked with 5% (v/v) non-fat milk (Beyotime, P0216) for 3 h. All primary antibodies were diluted in QuickBlock chemiluminescence detection system (SuperSignal West Pico PLUS Chemiluminescent Substrate), and immunoblotting images were collected using an eBlot (Genscript). Protein band intensities were analyzed and quantified using ImageJ software for grayscale measurements. The following antibodies were used: -Actin | Get A Quote |
Background Ultraviolet B (UVB) rays are a type of ultraviolet radiation emitted by the sun, primarily responsible for skin photodamage. These rays mainly affect the epidermis, leading to direct damage to DNA and contributing to skin cancer development. Retinol and its derivatives are effective in combating skin aging and photodamage, but they often cause skin intolerance, limiting their use despite their potent effects. Therefore, investigating optimal compositions of retinoids is essential to enhance their efficacy against photodamage.Method In this study, we investigated the synergistic effects of retinol (ROL) and retinyl palmitate (RPalm) in alleviating UVB-induced DNA damage in human keratinocytes (HaCaT) ... More
