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| Protein and Antibody Isolation | CM was then clarified by centrifugation at 1000 g for 10 min and filtered through a 0.22 m filter to remove residual debris. Protein A resin (1 mL, GenScript) was prepared by washing with 25 mL TBS in a gravity purification column. CM containing antigen-hIgG (50 250 mL) was passed through the resin by gravity | Get A Quote |
Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research and therapeutic applications. However, traditional nanobody generation methods rely on camelid immunization, which can be costly and time-consuming, restricting their practical feasibility. In this study, we present a phage-displayed synthetic library for nanobody discovery. To validate this approach, we screened nanobodies targeting various Drosophila secreted proteins. The nanobodies identified were suitable for applications such as immunostaining and immunoblotting, supporting the phage-displayed synthetic library as a ve... More
