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Constitutive arrestin recruitment by orphan GPR52 via an atypical binding mode

Cell Research . 2025-08; 
Xi Lin, Xiaohu Wei, Ning Pu, Ling Wang, Zhibin Zhang, Cuixia Li, Yang Yue, Junlin Liu, Qiwen Tan, Qianqian Sun, Fei Xu
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Proteins, Expression, Isolation and Analysis The supernatant was isolated by ultracentrifugation at 35,000 rpm at 4 °C for 30 min, and then incubated with High Affinity Ni-Charged Resin FF (Genscript, 2L biomass need 800 μL resin) and 20 mM imidazole at 4 ℃ for 3 h. Get A Quote

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