| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | Western Quick Block Kit (GenScript Corporation, China); The transfer current was set to a constant current, 400 mA, for 20 min. The membrane was blocked by Western Quick Block Kit (GenScript) on a room temperature shaker for T. Ma et al. Bioactive Materials 50 (2025) 171–188 173 10–20 min, washed with TBST, and then incubated with diluted primary antibody overnight at 4 ◦C. | Get A Quote |
The onset of periprosthetic osteolysis is mediated by wear particles following artificial arthroplasty. This manifests as a disturbed bone metabolism microenvironment, characterized by insufficient osteogenesis and angiogenesis, and enhanced osteoclastic activity. To target and remodel the homeostatic environment of bone metabolism in the sterile region around the prosthesis, we successfully pioneered the proposal and construction of a fused exosome (f-exo) system with M2 macrophage-derived exosomes (M2-exo) and urine-derived stem cell exosomes (USC-exo). The results demonstrate that f-exo effectively combines the osteolysis region-targeting capabilities of M2-exo with the bone metabolic homeostasis modulation ... More
