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Circular RNA circRNA_0082835 promotes progression and lymphatic metastasis of primary melanoma by sponging microRNA miRNA-429

Bioengineered. 2021-12; 
Yute Sun, Zuoqiong Hou, Binlin Luo, Chujun Li, Jinfang Liu, Jianlan Liu, Jian Tang, Gang Yao
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Proteins, Expression, Isolation and Analysis … SurePAGE™ precast sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel (GenScript, Nanjing, China) was used for electrophoresis. SDS-PAGE buffer was added to the protein samples, which were then denatured by boiling water bath at 100C for 5 min. … Get A Quote

摘要

To identify how circular RNA circRNA_0082835 impacts melanoma cells and lymphatic metastasis to observe whether it exerts effects through its action mechanism of sponging microRNA miR-429. Clinical baseline information was collected, and clinical samples were used for detection on circRNA_0082835 and EZH2. The expression of circRNA_0082835, EZH2, and miR-429 was detected by quantitative real-time PCR (RT-qPCR). Cell proliferation was tested with cell counting kit-8 (CCK-8). Flow cytometry was applied to examination of cell cycle levels. Cell invasion and migration were observed by transwell and wound healing. The expression of Wnt/β-catenin pathway, cell cycle and epithelial-mesenchymal transition (EMT) marker... More

关键词

CircRNA_0082835, EZH2, Wnt/β-catenin, miR-429, primary melanoma