Products/Services Used | Details | Operation |
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Catalog Peptides> | The adeno-associated virus (AAV) vectors were synthesised by Hanheng Biotechnology Co., Ltd. (Shanghai, China). To induce active EAU, B6 mice were injected subcutaneously with 200 g of IRBP (peptides 1?C20) (Genscript, Nanjing, China) | Get A Quote |
Uveitis is a disease resulting in the inflammation of uveal tracts, but the factors resulting in uveitis is still obscure. Previous studies have shown that miR-379-5p was involved in the pathogenesis of several diseases, however, the role and regulatory mechanism of miR-379-5p in uveitis were unclear. In our study, we established experimental autoimmune uveitis (EAU) mouse models to explore the role of miR-379-5p in uveitis. RT-qPCR identified that miR-379-5p level was increased in serum of EAU mice. In mechanism, SEMA3A 3'UTR was proven to be directly targeted by miR-379-5p and SEMA3A expression was negatively regulated by miR-379-5p in CD4 T cells. Moreover, ELISA analysis revealed that knockdown of miR-379-5... More