Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … of PCR amplified EF1α, PGK or UbC promoters in the previously built pLenti-GIII-CMV-mCherry plasmid, digested at either AvrII and NheI cutting sites for EF1α or cutting sites ApaI and BamHI for PGK and UbC, using a CloneEZ ® PCR Cloning Kit (GenScript #L00339) and … | Get A Quote |
In Duchenne muscular dystrophy (DMD) patients, absence of dystrophin causes muscle wasting by impacting both the myofiber integrity and the properties of muscle stem cells (MuSCs). Investigation of DMD encompasses the use of MuSCs issued from human skeletal muscle. However, DMD-derived MuSC usage is restricted by the limited number of divisions that human MuSCs can undertake in vitro before losing their myogenic characteristics and by the scarcity of human material available from DMD muscle. To overcome these limitations, immortalization of MuSCs appears as a strategy. Here, we used CDK4/hTERT expression in primary MuSCs and we derived MuSC clones from a series of clinically and genetically characterized patien... More