至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Sex diversity in proximal tubule and endothelial gene expression in mice with ischemic acute kidney injury

Clin Sci (Lond). 2020; 
Jose L Viñas, Christopher J Porter, Adrianna Douvris, Matthew Spence, Alex Gutsol, Joseph A Zimpelmann, Karishma Tailor, Pearl A Campbell, Kevin D Burns
Products/Services Used Details Operation
Molecular Biology Reagents … Tubular apoptosis was assayed using the TUNEL Apoptosis Detection Kit (Genscript, Piscataway, NJ, USA), as described [17,18]. Caspase-3 activity. Caspase-3 activity was measured on 5 mg of kidney tissue, using the Caspase-3 Assay Kit (Abcam) [17,18]. Immunoblots … Get A Quote

摘要

Female sex protects against development of acute kidney injury (AKI). While sex hormones may be involved in protection, the role of differential gene expression is unknown. We conducted gene profiling in male and female mice with or without kidney ischemia-reperfusion injury (IRI). Mice underwent bilateral renal pedicle clamping (30 min), and tissues were collected 24 h after reperfusion. RNA-sequencing (RNA-Seq) was performed on proximal tubules (PTs) and kidney endothelial cells. Female mice were resistant to ischemic injury compared with males, determined by plasma creatinine and neutrophil gelatinase-associated lipocalin (NGAL), histologic scores, neutrophil infiltration, and extent of apoptosis. Sham mice ... More

关键词

acute kidney injury, sexual dimorphism, transcriptome