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PTPN22 interacts with EB1 to regulate T-cell receptor signaling

FASEB J. 2020; 
Xiaonan Zhang, Yang Yu, Bin Bai, Tao Wang, Jiahui Zhao, Na Zhang, Yanjiao Zhao, Xipeng Wang, Bing Wang
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Molecular Biology Reagents … GACATGACATGCTGGCCTGCG‐3′) and control shRNA (5′‐TGGCATTGTCTTACCGCCTAT‐ 3′), or PTPN22‐shRNA (5′‐GUACGGACACCUGAAUCAUdTdT‐3′) and its respective control shRNA (5′‐TGGCATTGTCTTACCGCCTAT‐3′) (GenScript, Piscataway, NJ, USA … Get A Quote

摘要

The PTPN22 gene encoding the Lyp/Pep protein tyrosine phosphatase is a negative regulator of T-cell receptor (TCR) signaling. Recent studies have shown that phosphorylation of end-binding protein 1 (EB1) is associated with the TCR activation. In this study, using 2-hybrid and mass spectrometry analyses, we identified EB1 as a protein associated with PTPN22. Furthermore, we discovered that EB1 specifically bound to the P1 domain of PTPN22 by competing with CSK, and the variant PTPN22-R620W does not affect the association with EB1, which is instrumental with respect to the regulation of TCR signaling. In addition, PTPN22 dephosphorylates EB1 at tyrosine-247 (Y247), which decreases the expression of the T-cell act... More

关键词

TCR signaling pathway, autoimmune diseases, protein interactions, tyrosine phosphatase