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SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors

biorxiv. 2020; 
Chao Gao, Junwei Zeng, Nan Jia, Kathrin Stavenhagen, Yasuyuki Matsumoto, Hua Zhang, Jiang Li, Adam J Hume, Elke Mühlberger, Irma van Die, Julian Kwan, Kelan Tantisira, Andrew Emili, Richard D Cummings
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Molecular Biology Reagents … differences in glycosylation of S and the variant glycoforms might provide insights into disease severity and spread of the virus in COVID-19 patients … The reaction mixture was directly loaded on the 4–20% SDS-PAGE gel (GenScript) following addition of 4× laemmli loading buffer … Get A Quote

摘要

The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate that such receptors are highly expressed in innate immune cells in tissues susceptible to SARS-CoV-2 infection. Binding of the above receptors to S is characterized by affinities in the picomolar range and consistent with S glycosylation analysis demonstrating a variety of N- and O-glycans as receptor ligands. These results indicate multipl... More

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