| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | … differences in glycosylation of S and the variant glycoforms might provide insights into disease severity and spread of the virus in COVID-19 patients … The reaction mixture was directly loaded on the 4–20% SDS-PAGE gel (GenScript) following addition of 4× laemmli loading buffer … | Get A Quote |
The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate that such receptors are highly expressed in innate immune cells in tissues susceptible to SARS-CoV-2 infection. Binding of the above receptors to S is characterized by affinities in the picomolar range and consistent with S glycosylation analysis demonstrating a variety of N- and O-glycans as receptor ligands. These results indicate multipl... More
