至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Exosomes derived from miR-155-5p-overexpressing synovial mesenchymal stem cells prevent osteoarthritis via enhancing proliferation and migration, attenuating apoptosis, and modulating extracellular matrix secretion in chondrocytes

Cell Biol Toxicol. 2020; 
Zhirong Wang, Kai Yan, Gaoran Ge, Di Zhang, Jiaxiang Bai, Xiaobin Guo, Jing Zhou, Tianpeng Xu, Menglei Xu, Xiao Long, Yuefeng Hao, Dechun Geng
Products/Services Used Details Operation
Molecular Biology Reagents … Human osteoarthritic chondrocytes were cultured and transfected with miR-7a, miR-206, miR-320a, miR-155-5p mimic (GenScript; Nanjing, China), and miR-155-5p inhibitor (GenScript; Nanjing, China) at a concentration of 100 nM by Lipofectamine® 2000 transfection reagent … Get A Quote

摘要

Synovial mesenchymal stem cells (SMSCs) have the potential to attenuate osteoarthritis (OA)-induced injury. The role and mechanism of SMSC-derived exosomes (SMSC-Exos), pivotal paracrine factors of stem cells, in OA-associated injury remain unclear. We aimed to confirm the effect of SMSC-Exos with specific modifications on OA-induced damage and to investigate the potential molecular mechanisms. Exosomes derived from miR-155-5p-overexpressing SMSCs (SMSC-155-5p-Exos) and SMSCs (SMSC-Exos) were isolated and characterized. CCK-8, Transwell, and Western blot analyses were used to detect proliferation, migration, extracellular matrix (ECM) secretion, and apoptosis of osteoarthritic chondrocytes. The therapeutic effe... More

关键词

Exosomes, Osteoarthritis, Runx2, Synovial mesenchymal stem cells, miR-155-5p