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Molecular Biology Reagents> | … Search for other works by this author on: This Site. PubMed. Google Scholar. Hyun S. Lillehoj Hyun S. Lillehoj C. A Animal Biosciences and Biotechnology Laboratory, USDA-ARS, 10300 Baltimore … Mouse mAbs were produced by the described protocol (24) of GenScript Inc … | Get A Quote |
Necrotic enteritis (NE) is a devastating enteric disease caused by Clostridium perfringens type A/G, which affects global poultry industry by compromising the performance, health, and welfare of chickens. The causative main virulent factor responsible for NE pathogenesis has been shifted from a phospholipase C portion of an α-toxin, to an NE B-like (NetB) toxin, a plasmid-encoded pore-forming heptameric protein, in NE development. Therefore, the ability to detect NetB toxin will enable early diagnosis of field NE. Because the NetB protein can only be detected by western blot analysis with polyclonal anti-NetB antiserum, we developed a NetB-specific monoclonal antibody (mAb)-based capture enzyme-linked immunoso... More