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Custom Vector Construction> | … 3′ (bottom strand). Both strands were annealed and ligated into pRNAT-H1.1/Adeno (GenScript Biotech, Piscataway Township, NJ, USA) and digested with MluI and HindIII. Recombinant adenoviral vector was produced by … | Get A Quote |
Airway goblet cell differentiation and related mucus overproduction are critical processes in the development of respiratory diseases, including asthma. The underlying mechanisms, however, are not fully understood. We identified Runt-related transcription factor 2 (Runx2) as a novel regulator for goblet cell differentiation. Runx2 was up-regulated by 6.4-fold during IL-13-induced goblet cell differentiation of human bronchial epithelial cells. Knockdown of Runx2 attenuated the IL-13-induced differentiation/mucus production by 67%. Mechanistically, Runx2 bound to the promoter of SAM-pointed domain-containing Ets-like factor (SPDEF), a known factor for goblet cell differentiation, resulting in an activation of SP... More