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Genomic integration occurs in the packaging cell via unexported lentiviral precursors

Biotechnol Lett. 2016-06-01; 
Abdullah Al Mosabbir, Kevin Truong
Products/Services Used Details Operation
Custom Vector Construction … Vector construct and synthesis. DNA isolation and manipulations were performed according to Davis (2012). The vectors SINp-Cherry, GP, GP (NoMA), and GP (NoIN) were synthesized by Genscript (Pescataway, NY) and subcloned into the pUC57-Simple vector using EcoRV … Get A Quote

摘要

objective: To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection. results: A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag-pol (GP) and a transfer vector, without the envelope vector, produces a stable cell line after 2 weeks of selection. Furthermore, a matrix protein deficient GP in the packaging vector enhances this integration. This supports that, in theory, unexported lentiviral cores produced within the packaging cell can infect itself without requiring the release of any lentiviral particles. conclusions: If the packaging cell is also the target cell, then gene i... More

关键词

Gene integration, Human embryonic kidney cell, Lentivirus, Stable cell line