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Adenovirus vectors can induce activation of endothelial cells: CD40-CD40L interactions partly participate in the endothelial cells activation induced by adenovirus vectors in an NF-kappaB-dependent manner.

Afr J Biotechnol.. 2011-10;  10(63):13705-13
Zhao-Lun Li, Wu-Jun Xue, Pu-Xun Tian, Tie Chong, Wei-Min Gan, Zhi-Ming Wang. Department of Urology, the Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, PR China.
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摘要

Replication-defective adenovirus vector without both E1 and E3 is one of the most popular tools in transgenic therapies. However, more attention should be paid to adenovirus vectors mediated-gene modified study on endothelial cells (ECs). To verify the possible danger in that process, we explored the effect of adenovirus on ECs in this study. By using western blot analysis, we showed that the level of both CD40 and CD40L on human umbilical vein endothelial cells (HUVECs) were upregraduated by adenovirus vector infection at 100 multiplicity of infection (MOI). The activation of ECs induced by adenovirus vector infection at MOI 100 can be partly inhibited by a blockade of CD40/CD40L interactions by using the reco... More

关键词

Adenovirus vector; CD40; CD40L; endothelial cells; NF-kappaB.