| Products/Services Used | Details | Operation |
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| Gene Synthesis | For PPT-resistant strain screening, the marine bacterium, isolated from deep sea mud samples provided by Third Institute of Oceanography in China, were cultured at 28 °C in M9 minimal medium supplemented with 0.4% (w/v) glucose as a carbon source and 50 mM PPT. After 5 to 7 days, colonies of the strains that grew rapidly were collected and further verified on M9 plates in the presence of 100 mM glyphosate PPT. After two round screening, one strainYM12 with high PPT-resistant obtained and its genomic DNA was prepared according to the methods described elsewhere [15]. To identify the strain, the 16S rRNA gene was amplified from the genomic DNA with the primers 16SF (5′-AGAGTTTGATCMT GGCTCAG-3′) and 16SR (5′-TACGGYTACCTTGTTACGACTT-3′), and the PCR fragments were sequenced by Genscript (Nanjing, China). | Get A Quote |
Phosphinothricin (PPT) is a kind of non-selective, environmentally friendly herbicide. PPT-tolerance genes are vital in both plant biotechnology as selectable markers and the development of transgenic herbicide-resistant crops. However, there are no other well-identified and commercially available PPT-resistance genes for use in plant genetic engineering besides two PPT N-acetyltransferase genes, which known as pat and bar derived from Streptomyces sp. Here, we isolated a novel PPT N-acetyltransferase gene from PPT-resistant marine bacteria, Rhodococcus sp. strain YM12. The gene, designated as RePAT, encoded a protein (RePAT) of 162 amino acids, which showed 37% identity with that of PAT proteins.... More
