| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | All the Taq DNA polymerases, T4 DNA ligases, restriction enzymes, and DNA markers were purchased from TaKaRa Company (Dalian, China). The primers and fragments were synthesized and sequenced, respectively, by GenScript Co. (Nanjing, China). The protein molecular weight marker was purchased from TIANGEN Co. (Germany) and THFA was synthesized in our laboratory as described by Scrimeour and Vitols [23]. The Kits including DNA purification, Protein Quantification and the GST Bind Purification were purchased from Axygen Co. (USA), Pharmacia Co. (Sweden) and Sangon Biotech Co. (China), respectively. Unless otherwise stated, all the other chemical materials used in this study were purchased from Sigma Company (USA). | Get A Quote |
SHMTserine hydroxymethyltransferasePLPpyridoxal 5′-phosphateTHFtetrahydrofolateGDCglycine decarboxylaseLBLuria-BertaniIPTGisopropyl-β-d-1-thiogalactopyranosideGSTglutathione S-transferaseSDS-PAGEsodium dodecyl sulfate polyacrylamide gel electrophoresisCTABcetyltrimethyl ammonium bromideRP-HPLCreversed phase high performance liquid chromatographyORFopening reading frameOPAorthophthalaldehydeA novel glyA gene from the marine bacterium Alcanivorax sp. was cloned and expressed in Escherichia coli BL21 (DE3). The recombinant glyA encodes a polypeptide of 418 amino acids, which was designated as AdSHMT that shows the highest identity (70%) with a SHMT from Shewanella algae. The purified enzyme showed a single band... More
