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Strain Relevant genotype and additional characteristics

semanticscholar.. 2017; 
D Chattoraj
Products/Services Used Details Operation
Custom Vector Construction pUC57-kan Cloning vector kanamycin GenScript, Piscataway Township, NJ, USA Get A Quote

摘要

Fluorescence-based methods are widely used to analyze elementary cell processes such as DNA replication or chromosomal folding and segregation. Labeling DNA with a fluorescent protein allows the visualization of its temporal and spatial organization. One popular approach is FROS (fluorescence repressor operator system). This method specifically labels DNA in vivo through binding of a fusion of a fluorescent protein and a repressor protein to an operator array, which contains numerous copies of the repressor binding site integrated into the genomic site of interest. Bound fluorescent proteins are then visible as foci in microscopic analyses and can be distinguished from the background fluorescence caused by unbo... More

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