| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Porcine skin gelatin, Captopril, formic acid, sodium tetraborate decahydrate (borax), MS-grade water and MS-grade acetonitrile were from Sigma Aldrich (Wicklow, Ireland). The enzyme preparation Brewer’s Clarex™ (specific activity: 37 × 10−3 U/mg; DSM, Heerlen, Netherlands) was kindly provided by the manufacturer. o-Aminobenzoylglycl-ρ-nitro-L-phenylalanyl-L-proline (Abz-Gly-Phe-(NO2)-Pro) and o-aminobenzoylglycine (Abz-Gly-OH) were from Bachem Feinchemikalien (Bubendorf, Switzerland). Trinitrobenzenesulfonic acid (TNBS) was from the Medical Supply Co. (Dublin, Ireland). Bovine lung was kindly supplied by a local abattoir (Gaelic Meat and Livestock Ltd., Limerick, Ireland). All other general laboratory chemicals were of analytical grade and were from Sigma Aldrich (Wicklow, Ireland). All synthetic peptides were from Genscript (New Jersey, USA). | Get A Quote |
A targeted enzymatic approach was employed for the generation of angiotensin converting enzyme (ACE) inhibitory/anti-hypertensive peptides. Porcine skin gelatin was hydrolysed with Aspergillus niger prolyl endoproteinase (An-PEP) to produce an hydrolysate with potent ACE inhibitory activity (mean IC50: 220.2 µg mL−1) after 4-h hydrolysis. Peptide identification was achieved by UPLC-ESI-MS/MS. The ACE-inhibitory activity of a selection of the identified peptides was determined. The most potent peptide was Met-Gly-Pro with an ACE IC50 of 51.11 ± 1.14 µM. Furthermore, oral administration (50 mg/kg body weight) of the hydrolysate to spontaneously hypertensive rats resulted in decreases in systolic and... More
