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In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis

Proceedings of the National Academy of Sciences. 2016;

Klara Kirschbauma,b,1, Jana K. Sonnera,1, Matthias W. Zellerc,d, Katrin Deumelandta , Julia Bodee,f, Rakesh Sharmae,f, Thomas Krüwele,f, Manuel Fischerb , Angelika Hoffmannb , Milene Costa da Silvag,h,i, Martina U. Muckenthalerg,h, Wolfgang Wickj,k, Björn Tewse,f, John W. Chenc,d, Sabine Heilandb , Martin Bendszusb , Michael Plattena,j, and Michael O. Breckwoldta,b,

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Peptide Synthesis	EAE was induced by s.c. injecting 200 μg proteolipid protein (PLP) peptide (PLP139–151; GenScript), emulsified in 50 μL PBS and 50 μL of complete Freund’s adjuvant (BD Difco, Fisher Scientific) containing 4 mg/mL heatinactivated Mycobacterium tuberculosis H37 Ra (BD Difco, Fisher Scientific). Two hundred nanograms of pertussis toxin (PTx) (List Biological Laboratories) dissolved in 200 μL PBS was administered i.p. on days 0 and 2. T	Get A Quote