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A population of proinflammatory T cells coexpresses αβ and γδ T cell receptors in mice and humans

J Exp Med. 2020-05;

Edwards SC, Sutton CE, Ladell K, Grant EJ, McLaren JE, Roche F, Dash P, Apiwattanakul N, Awad W, Miners KL, Lalor SJ, Ribot JC, Baik S, Moran B, McGinley A, Pivorunas V, Dowding L, Macoritto M, Paez-Cortez J, Slavin A, Anderson G, Silva-Santos B, Hokamp K, Price DA, Thomas PG, McLoughlin RM, Mills KHG.

Products/Services Used	Details	Operation
Catalog Peptides	For DC co-culture experiments, bone marrow from C57BL/6 mice was incubated with GM-CSF (20 ng/ml) for 6 d to generate mature DCs. Mature DCs were washed and incubated for 5 h at 20,000 cells/well (for culture with magnetically enriched γδ T cells) or 2,500 cells/well (for culture with flowpurified populations) with medium alone (negative control), OVA257–264 (4 µg/ml; Sigma-Aldrich), OVA323–339 (10 µg/ml; Sigma-Aldrich), KLH (10 µg/ml; Calbiochem), or MOG35–55 (10 µg/ml; GenScript).	Get A Quote

摘要

T cells are classically recognized as distinct subsets that express αβ or γδ TCRs. We identify a novel population of T cells that coexpress αβ and γδ TCRs in mice and humans. These hybrid αβ-γδ T cells arose in the murine fetal thymus by day 16 of ontogeny, underwent αβ TCR-mediated positive selection into CD4+ or CD8+ thymocytes, and constituted up to 10% of TCRδ+ cells in lymphoid organs. They expressed high levels of IL-1R1 and IL-23R and secreted IFN-γ, IL-17, and GM-CSF in response to canonically restricted peptide antigens or stimulation with IL-1β and IL-23. Hybrid αβ-γδ T cells were transcriptomically distinct from conventional γδ T cells and displayed a hyperinflammatory phenotyp... More