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Molecular evolution of luciferase diversified bioluminescent signals in sea fireflies (Crustacea: Ostracoda: Cypridinidae)

biorxiv. 2020; 
Nicholai M. Hensley,  Emily A. Ellis,   Nicole Y. Leung,  John Coupart,  Alexander Mikhailovsky,  Daryl A. Taketa,   Michael Tessler,   David F. Gruber,  Anthony W. De Tomaso,  Yasuo Mitani,  Trevor J. Rivers,   Gretchen A. Gerrish,  Elizabeth Torres,  Todd H. Oakley
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Molecular Biology Reagents The luciferase genes of P. morini and M. sp. SVU were synthesized and cloned into the mCherry-C vector by Genscript (Piscataway, New Jersey, USA) with flanking restriction enzyme sites. Get A Quote

摘要

Integrative studies of courtship signals provide rich opportunities to understand how biological diversity originates, but understanding the underlying genetics is challenging. Here we show molecular evolution of a single gene - luciferase - influenced diversification of bioluminescent signals in cypridinid ostracods, including their radiation into dozens of Caribbean species with distinctive courtship displays. We report emission spectra from twenty-one species, thirteen luciferases from transcriptomes, and in vitro assays that confirm function from four exemplar species. We found most sites in luciferase evolved neutrally or under purifying selection, including multiple sites that affect the color of biolum... More

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