至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Unique structural features of mammalian NEIL2 DNA glycosylase prime its activity for diverse DNA substrates and environments

biorxiv. 2020; 
Brian E. Eckenroth,  Vy Cao,  April M. Averill,  Julie A. Dragon, Sylvie Doublié
Products/Services Used Details Operation
Molecular Biology Reagents Both full-length and truncated gray short tailed opossum Monodelphis domestica Neil2 (MdoNEIL2) sequences were synthesized and codon-optimized for expression in E. coli by GenScript and subcloned into a pET30 vector (Novagen) using NdeI and XhoI restriction sites, then transformed into BL21(DE3) plated onto LB-agar under kanamycin selection. Get A Quote

摘要

Oxidative damage on DNA arising from both endogenous and exogenous sources can result in base modifications that promote errors in replication as well as generate sites of base loss (abasic sites) that present unique challenges to maintaining genomic integrity. These lesions are excised by DNA glycosylases in the first step of the base excision repair pathway. Here we present the first crystal structure of a NEIL2 glycosylase, an enzyme active on cytosine oxidation products and abasic sites. The structure reveals an unusual “open” conformation not seen in NEIL1 or NEIL3 orthologs. NEIL2 is predicted to adopt a “closed” conformation when bound to its substrate. Combined crystallographic and solution scat... More

关键词

DNA damage, DNA repair, NEIL2 glycosylase, SAXS, crystallography