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Characterization and Expression Profiling of Recombinant Parathyroid Hormone (rhPTH) Analog 1–34 in Escherichia coli, Precise with Enhanced Biological Activity

International Journal of Peptide Research and Therapeutics. 2020; 
Rajeenkanna Chilakapati  · Chanchal Thomas Mannully  · Mrinmoy Ghosh  · K. K. Pulicherla
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摘要

Human truncated parathyroid hormone [hPTH (1–34)], a peptide hormone which accelerated the research interest towards the theraputical applications. This study outlines the effective expression of [hPTH (1–34)] in Escherichia coli and the recuperation of highly soluble truncated PTH from the fusion protein by proteolytic digestion. Successful expression of glutathione S-transferase (GST) fusion protein was achieved by incorporating truncated PTH with an enzymatic cleavage site into the “N” terminal GST of pGEX-4T-3 expression vector. Under the optimized condition, we achieved more than 120 mg of pure hPTH (1–34) per liter of bacterial culture, with an overall yield of 39%. Purification process was car... More

关键词

Truncated parathyroid hormone · Escherichia coli · Glutathione S-transferase · Enterokinase · Purification