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| Molecular Biology Reagents | One μg of each DNA sample was digested with 5 units of EcoR1 and 5 units of Mse1enzymes. The digested DNA samples were incubated at 37o C for 3½ hours. EcoR1adaptor (10 pmol μl-1), Mse1 adaptor (10 pmol μl-1), 5 units of T4 DNA ligase were added to the double digested DNA samples and were incubated overnight (~16 hours) at 37o C (Rajkumar et al., 2011). Subsequently, 5 μl of each digested/ ligated DNA samples were pre-amplifi ed in 25 μl reaction volume containing 2 μl of each preamplifi cation primers (8 pmol μl-1), dNTPs (0.2 mM ), 1X PCR buff er (Genscript, USA) and 1 unit of Taq DNA polymerase (5 units μl- 1) (Genscript, USA) (Ekanayake et al., 2016). PCR amplifi cation was performed with the following temperature profi le: 30 cycles of denaturation at 94o C for 30 s, annealing at 56o C for 60 s, extension at 72o C for 60 s (Rajkumar et al., 2011; Ekanayake et al., 2016). | Get A Quote |
Purpose: Herbicide-resistant rice varieties in combination with post-emergent broad-spectrum herbicides improve the eff ectiveness of weed management in rice fi elds. Herbicide-resistance can be acquired naturally or induced by classical or modern breeding techniques. In the present study, screening of Sri Lankan rice varieties to glufosinate was examined and attempts were made to develop glufosinate-resistant rice varieties with the help of the breeding technique, mutagenesis. Research Method: Natural glufosinate-resistance was studied in seven (7) traditional and eighteen (18) inbred rice varieties under 0.27, 0.30 and 0.33 kg ha -1 glufosinate concentrations. Induced glufosinateresistance in seed-derived cal... More
