| Products/Services Used | Details | Operation |
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| Gene Synthesis | Bacterial genomic DNA was isolated using the method of Berg et al. (2009). Microbial 16S rRNA gene was amplified with the universal primers pair 27 F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492 R (5′-TACGGCTACCTTGTTACGACTT-3′) (Weisburg et al. 1991). The amplification was performed with an initial denaturation step of 94 °C for 1 min, followed by 30 cycles of 30 s at 94 °C, 30 s at 50 °C and 2 min at 72 °C, plus a final extension at 72 °C for 7 min. After being checked using 1% agarose gel electrophoresis, PCR products were purified using the SanPrep Column PCR Product Purification Kit (Sangon Biotech, Shanghai, China) and sequenced by GenScript Corporation Ltd. (Nanjing, China). The nucleotide sequences obtained in this study were deposited in GenBank under the accession numbers as shown in Table 1, and were compared to known 16S rRNA gene sequences in the GenBank database using BLAST to find the closest relatives. | Get A Quote |
The interactions between bacteria and algae may play a significant part in the formation and development of algal blooms. The bloom-forming cyanobacterium Microcystis occurs mainly as colonial form in natural waters, and thus it is necessary to study the interaction between bacteria and colonial Microcystis. This paper aimed to investigate effects of the cultivable bacteria attached to Microcystiscolonies on the colony size and growth of colonial Microcystis aeruginosa. Eleven bacterial strains were isolated from M. aeruginosa colonies collected from Lake Taihu. Among these bacteria, seven bacterial isolates significantly influenced the colony size of M. aeruginosa, and four bacterial isolates signifi... More
