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Mitochondrial genome of Lemnia saucia Mulsant (Coleoptera: Coccinellidae) and phylogenetic analysis

Journal Mitochondrial DNA Part B. 2019; 
Jing Zhou, Ran Li, Yong Kong, Hao-Wu Hu & Xiao-Han Shu Lanxi Plant Protection and Quarantine Station, Lanxi, PR China Correspondence xiaohanshu@aliyun.com View further author information
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Gene Synthesis Then PCR products were sequenced using primer-walking strategy from both strands by Genscript Biotech Corp. (Nanjing, China). Mitochondrial genome was assembled by SeqMan program from DNASTAR (Burland 2000) and annotated using MITOS Web Server (Bernt et al. 2013). Get A Quote

摘要

We sequenced and annotated the nearly complete mitochondrial genome (mitogenome) of Lemnia saucia (Coleoptera: Coccinellidae). This mitogenome was 14,106 bp long and encoded 13 protein-coding genes (PCGs), 19 transfer RNAgenes, and two ribosomal RNA genes. The overall nucleotide composition was 41.6% of A, 8.8% of G, 37.3% of T, and 12.4% of C. Phylogenetic reconstruction using both Bayesian inference (BI) and maximum likelihood (ML) validated the taxonomic status of L. saucia, exhibiting the close relationship with Propylea japonica.

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