| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | The PCR reaction was performed in 25 μl containing 1 μl of cDNA template, 1 μl of each primer, 12.50 μl rTaq poly mixture and 9.50 μl distilled water. PCR conditions were: initial denaturation at 94 °C for 3 min; 34 cycles of 94 °C 30s, 58 °C for 1 min, 72 °C for 1 min; final extension at 72 °C for 10 min. The PCR product was analyzed by 1.2% agarose gel and ligated into pEASY T3 cloning vector (TransGen, Beijing, China) for 5 min at 25 °C. Products were transformed into Trans-T1 competent cells with the addition of IPTG and Xgal, plating in LB ampicillin and were incubated overnight. The positive clones were selected and confirmed by sequencing (GenScript Biology Company, Nanjing, China). | Get A Quote |
Odorant sensitivity and selectivity play a crucial role in insects for finding host plants, mates and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in the detection of general odorants released by foods and host plants. However, recent studies suggest GOBPs may also play some role in detection of sex pheromones. In the present study with the rice striped stem borer Chilo suppressalis (Walker) real time quantitative PCR (qPCR) measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. To examine their specific functions, binding affinities to three sex pheromones and 35 plant v... More
