| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The partial PxylGR43a-2 sequence from our transcriptome data was different to NCBI reported PxylGR43a-1 at 5′ end. RACE PCRs were conducted to obtain full length sequence using the SMART™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) according to the manufacturer's instructions. The 5′ and 3′ RACE primers were designed by Primer Premier 5 and shown in Table S1. The RACE PCR products were subcloned into the pEASY-T3 cloning vector system (TransGene Biotech, Beijing, China) and positive clones were sequenced by GenScript (Nanjing, China). Full length PxylGR43a-2 sequence was determined by assembling the cDNA fragments from the 5′ and 3′ RACE PCRs, and confirmed by the end-to-end PCR. | Get A Quote |
Insect gustatory system plays important roles in multiple behaviors including feeding, mating, and oviposition. Gustatory receptors (GRs), located on the dendritic membrane of gustatory sensory neurons (GSNs), are crucial in peripheral coding of non-volatile compounds. However, GRs and their detailed functions remain poorly understood in lepidopteran pests. In the present work, focusing on GR genes of Plutella xylostella, an important worldwide crop pest, we cloned a candidate fructose GR gene that has two spliced variants (PxylGR43a-1 and PxylGR43a-2), and determined the tissue expression profiles by semi-quantitative reverse transcription PCR (RT-PCR). It revealed that both GR variants were highly expresse... More
