| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The mRNA primer sequences (GenScript, Nanjing, China) used for RT–qPCR were mouse PKIα: 5′-AGAGAAGC TCCACCGAACAA-3′ (forward, F), 5′-TGGCAACCAACA GTGTCTTG-3′ (reverse, R); human PKIα: 5′-GTGTGG TTGTGCCAGAAACT-3′ (F), 5′-GCAACCATGCCCTTA TTCCA-3′ (R); human IL-33: 5′-CGGTGTTGATGGT AAGATG-3′ (F), 5′-AGAGTGTTCCTTGTTGTTG-3′ (R); mouse g lyceraldehyde 3-phosphate dehydrogenase (GAPDH): 5′-GGTTGTCTCCTGCGACTTCA-3′ (F), 5′-TGGTCCAGGGTTTCTTACTCC-3′ (R) ; human GAPDH: 5′-CTTCTTTTGCGTCGCCAGCCGA-3′ (F), 5′-ACCAGGCGCCCAATACGACCAA-3′ (R) . | Get A Quote |
Recent studies have demonstrated that microRNA-155-5p (miR-155-5p) plays an essential role in the regulation of allergen-induced inflammation and is overexpressed in the skin of patients with atopic dermatitis (AD), although the mechanism is unknown. In this study, silencing miR-155-5p attenuated the thickening of the epidermis in AD model and reduced the infiltration of inflammatory cells and the secretion of Th2 cytokines. Protein kinase inhibitor α (PKIα) was identified as a direct target of miR-155-5p and correlated negatively with miR-155-5p in our AD model. Fluorescence in situ hybridization showed that miR-155-5p-expressing cells were predominantly present in the epidermis. When epithelial cells were t... More
