| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … transformation method, the natural transformation induced by CSP, was performed as previously described (Tabata et al., 2014); log-phase cells were incubated with 5–10 μg/mL of CSP (sequence: DSRIRMGFDFSKLFGK, synthesized by GenScript, Piscataway, NJ, USA) and … | Get A Quote |
Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence of MGEs in Streptococcus anginosus subsp. anginosus (SAA), a representative species belonging to AGS, and found a novel plasmid from SAA strain 0430-08. This plasmid was 7038bp and ~31% G/C content which we named pSAA0430-08, and examined its genetic structure and characteristics. Open reading frame (ORF) prediction revealed that pSAA0430-08 was composed of 10 ORFs including a putative plasmid repl... More
