| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | The PCR was performed under the following programs: incubation at 94 °C for 3 min, followed by 35 cycles at 94 °C for 30 s, 55 °C for 30 s, 72 °C for 90 s, and then extension at 72 °C for 2 min. The PCR product was subcloned into the pMD19-T vector (TaKaRa, Dalian, China), and the plasmids were sequenced (Genscript, Nanjing, China). | Get A Quote |
Postharvest storage conditions affect the ascorbic acid (AsA) levels in fresh-cut leaves of horticultural crops. However, the detailed mechanism of AsA metabolism in the fresh-cut leaves of tea plant (Camellia sinensis) during postharvest storage under light/dark conditions remains unclear. To investigate the AsA mechanism, we treated fresh-cut tea leaves with light/dark during postharvest storage. An ascorbate peroxidase 1 (CsAPX1) protein involved in AsA metabolism was identified by iTRAQ analysis. Gene expression profile of CsAPX1 encoding ascorbate peroxidase (APX) was regulated by light/dark conditions. AsA accumulation and APX activity were suppressed by light/dark conditions. SDS-PAGE analysis showed t... More
