| Products/Services Used | Details | Operation |
|---|---|---|
| Nucleic Acid Purification & Analysis | … PrimerQuest/Home/Index. Online design tools also exist for TaqMan probes (https://www.genscript.com/ssl-bin/app/primer). 45. Alternatively, run 5 μL from each PCR reaction in a 3 % agarose gel to check specificity. 46. Many biotech … | Get A Quote |
Atherosclerosis involves changes in gene and protein expression patterns in affected arteries. Quantification of these alterations is essential for understanding the molecular mechanisms underlying this pathology. Western blot and real-time PCR-used to quantify protein and messenger RNA levels, respectively-are invaluable molecular biology tools, particularly when material is limited. The availability of many genetically modified mouse models of atherosclerosis makes the mouse aorta an ideal tissue in which to carry out these expression pattern analyses. In this chapter, protocols are presented for mRNA and protein extraction from mouse aorta and for the accurate quantification of mRNA expression by RT-PCR and ... More
