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Genome Analysis of Cytochrome in Dinotefuran-Treated Apolygus lucorum (Meyer-Dür).

Bull Environ Contam Toxicol. 2019;

Wu S,, Deng D, Jiang W, Zhang K, Guo J, Duan W, Wang H.

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DNA Sequencing	… RACE). Outer and Inner reverse primers were designed (Table 1) and used to amplify the 5′ cDNA encoding for P450. Several independent subclones were sequenced by GenScript (Nanjing) Co., Ltd. (Nanjing, China). MEGA7 …	Get A Quote

摘要

In this study, two CYP genes, CYP395G1 and CYP4EY1, were analyzed in Apolygus lucorum (Hemiptera: Miridae). The expression pattern in different developmental stages of both CYP395G1 and CYP4EY1 revealed that first instar nymphs possessed the highest gene-transcript levels. After 12 h of dinotefuran treatments, the expression levels of CYP395G1 increased by 1.92-fold, while the CYP4EY1 expression decreased. It was observed that the sensitivities of dinotefuran to laboratory strains from F0 to F9 and the mRNA expression levels of CYP395G1 in the F9 dinotefuran selected strain were higher when compared to the control strain. The results suggest that CYP395G1 was possibly a candidate P450 that was involved in dino... More