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Molecular identification and detection of Wohlfahrtia magnifica in ovine vulvar myiasis in Gansu, China.

Trop Anim Health Prod. 2019;

Li Y, Li X, Liu J, Liu A, Guo P, Han Y, Shang Y, Guan G, Liu Z, Liu G,, Luo J,, Yin H,.

Products/Services Used	Details	Operation
Gene Synthesis	… Trop Anim Health Prod Page 3. of three genes were sequenced by GenScript Corporation (Nanjing, NJ, China). Basic Local Alignment Search Tool software (http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD= Web&PAGE_TYPE= BlastHome) was used for sequence analysis …	Get A Quote

摘要

Traumatic myiasis causes substantial economic losses to farmers worldwide. In the present study, six flocks of sheep (2261 sheep) were investigated in Gansu, China, and 207 of 552 larvae were genetically characterized based on three genes, including cyt b, EF-1α, and white gene, by polymerase chain reaction and sequence analysis. A survey of sheep in China revealed that the prevalence of vulvar myiasis of six sheep flocks was 5.00% (11/220, Flock1), 4.85% (10/206, Flock2), 4.50% (9/200, Flock3), 5.00% (15/300, Flock4), 4.68% (15/320, Flock5), 0% (0/1015, Flock6), respectively. The sequence and phylogenetic analysis showed that only Wohlfahrtia magnifica was detected in the field samples. This is the first repo... More