| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Some commonly used restriction enzyme sites were then added in-between or around the genes to facilitate subsequent cloning steps (Figure 1). The two genes with suitable promoters were then synthesized commercially (Genscript, USA) and ligated to a standard vector … | Get A Quote |
Ostreococcustauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13-22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave >104 transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a f... More
