| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | For peptide-specific cytokine production, cells were restimulated with M133 peptide (TVYVRPIIEDYHTLT; GenScript) in the presence of brefeldin A (5 µg/ml) for 4 h at 37 °C. | Get A Quote |
Fibroblastic reticular cells (FRCs) of secondary lymphoid organs form distinct niches for interaction with hematopoietic cells. We found here that production of the cytokine IL-15 by FRCs was essential for the maintenance of group 1 innate lymphoid cells (ILCs) in Peyer's patches and mesenteric lymph nodes. Moreover, FRC-specific ablation of the innate immunological sensing adaptor MyD88 unleashed IL-15 production by FRCs during infection with an enteropathogenic virus, which led to hyperactivation of group 1 ILCs and substantially altered the differentiation of helper T cells. Accelerated clearance of virus by group 1 ILCs precipitated severe intestinal inflammatory disease with commensal dysbiosis, loss of in... More
